Rice Starch Quality Improvement by Transgenic Technology

doi:10.3969/j.issn.1006-8082.2018.01.001

Abstract

Abstract: Starch is the main form of nutrition and energy storage of rice grains, which is mainly composed of amylose and amylopectin. Improvement of quality of rice starch could be modified through changing the content and quality of starch by transgenic technology. The practical cases of starch quality improvement in transgenic rice of therelated starch synthesizing genes were reviewed in this paper.

Key words: rice, starch, transgenic technology

摘要： 淀粉是稻谷籽粒主要的营养和能量储藏形式，主要由直链淀粉和支链淀粉组成。水稻淀粉品质的改良可以利用转基因技术修饰淀粉含量与质量的变化。本文综述了转淀粉合成相关基因改良水稻淀粉品质的实际案例。

关键词: 水稻, 淀粉, 转基因技术

CLC Number:

S511

丁一,丁箐雯,李冰清,沈易,张宁*,吴殿星. 转基因技术改良水稻淀粉品质研究进展[J]. 中国稻米, DOI: 10.3969/j.issn.1006-8082.2018.01.001 .

References

[1] Smith A M,K Denyer A, Martin C. The synthesis of the starch granule[J]. Annu Rev Plant Physiol Plant Mol Biol, 1997, 48: 65-87. [2] Zeeman S C, Kossmann J, Smith A M. Starch: Its metabolism, evolution, and biotechnological modification in plants[J]. Annu Rev Plant Biol, 2010, 61: 209-234. [3] Fujita N. Starch Biosynthesis in rice endosperm[J]. Agri-Bioscience Monographs（AGBM）, 2014, 4（1）: 1-18. [4] Bajaj S, A Mohanty. Recent advances in rice biotechnology-towards genetically superior transgenic rice[J]. Plant Biotechnol J, 2005, 3（3）: 275-307. [5] Sakulsingharoj C, Choi S B, Hwang S K, et al. Engineering starch biosynthesis for increasing rice seed weight: the role of the cytoplasmic ADP-glucose pyrophosphorylase[J]. Plant Sci, 2004, 167（6）: 1 323-1 333. [6] 宋敏，李援亚，张云孙.导入AGPase基因的转基因可育水稻及其经济性状的研究[J]. 华北农学报，2001，16（4）：11-14. [7] Fasahat P, Rahman S, Ratnam W. Genetic controls on starch amylose content in wheat and rice grains[J]. J Genet, 2014, 93（1）: 279-292. [8] Shimada H, Tada Y, Kawasaki T, et al. Antisense regulation of the rice waxy gene-expression using a pcr-amplified fragment of the rice genome reduces the amylose content in grain starch[J]. Theor Appl Genet, 1993, 86（6）: 665-672. [9] Terada R, Nakajima M, Isshiki M, et al. Antisense Waxy genes with highly active promoters effectively suppress waxy gene expression in transgenic rice[J]. Plant Cell Physiol, 2000, 41（7）: 881-888. [10] Itoh K, Ozaki H, Okada K, et al. Introduction of Wx transgene into rice wx mutants leads to both high- and low-amylose rice[J]. Plant Cell Physiol, 2003, 44（5）: 473-480. [11] Shimada H, Tada Y, Kawasaki T, et al. Antisense regulation of the rice waxy gene expression using a PCR-amplified fragment of the rice genome reduces the amylose content in grain starch[J]. TheorAppl Genet, 1993, 86（6）: 665-672. [12] 陈秀花，刘巧泉，王兴稳，等. 反义Wx基因导入我国籼型杂交稻重点亲本[J]. 科学通报，2002，47（9）：684-688. [13] Zeng D, Yan M, Wang Y, et al.Du1, encoding a novel Prp1 protein, regulates starch biosynthesis through affecting the splicing of Wx（b）supercript stop pre-mRNAs in rice （Oryza sativa L.）[J]. Plant MolBiol, 2007, 65（4）: 501-509. [14] 胡昌泉，徐军望，苏军，等. 农杆菌介导法获得转可溶性淀粉合成酶基因籼稻[J]. 福建农业学报，2003，18（2）：65-68. [15] Naoko F, Isao H, Sachi S, et al. Elongated phytoglycogen chain length in transgenic rice endosperm expressing active starch synthase IIa affects the altered solubility and crystallinity of the storage alpha-glucan[J]. J Exp Bot, 2012, 63（16）: 5 859-5 872. [16] Liu Q, Wang Z, Chen X, et al. Stable inheritance of the antisense Waxy gene in transgenic rice with reduced amylose level and improved quality[J]. Transgenic Res, 2003, 12（1）: 71-82. [17] Crofts N, Abe K, Aihara S, et al. Lack of starch synthase IIIa and high expression of granule-bound starch synthase I synergistically increase the apparent amylose content in rice endosperm[J]. Plant Sci, 2012, 193: 62-69. [18] Hanashiro I, Itoh K, Kuratomi Y, et al. Granule-bound starch synthase I is responsible for biosynthesis of extra-long unit chains of amylopectin in rice[J]. Plant Cell Physiol, 2008, 49（6）: 925-933. [19] Tanaka N, Fujita N, Nishi A, et al. The structure of starch can be manipulated by changing the expression levels of starch branching enzyme llb in rice endosperm[J]. Plant Biotechnol J, 2004, 2（6）: 507-516. [20] Butardo V M, Fitzgerald M A, Bird A R, et al. Impact of down-regulation of starch branching enzyme IIb in rice by artificial micro RNA- and hairpin RNA-mediated RNA silencing[J]. J Exp Bot, 2011, 62（14）: 4 927. [21] Zhu L J. High-amylose rice improves indices of animal health in normal and diabetic rats[J]. Plant Biotechnol J, 2012, 10（3）: 353-362. [22] Zhu L J, Liu Q Q, Wilson J D, et al. Digestibility and physicochemical properties of rice （Oryza sativa L.） flours and starches differing in amylose content[J]. Carbohydrate Polymers, 2011, 86（4）: 1 751 -1 759. [23] Wei C, Qin F, Zhou W, et al. Comparison of the crystalline properties and structural changes of starches from high-amylose transgenic rice and its wild type during heating[J]. Food Chem, 2011, 128（3）: 645-652. [24] Wei C, Qin F, Zhu L, et al. Microstructure and ultrastructure of high-amylose rice resistant starch granules modified by antisense RNA inhibition of starch branching enzyme[J]. J Agric Food Chem, 2010, 58（2）: 1 224-1 232. [25] Man J, Yang Y, Zhang C, et al. Structural changes of high-amylose rice starch residues following in vitro and in vivo digestion[J]. J AgricFood Chem, 2012, 60（36）: 9 332-9 341. [26] Qin F, Man J, Xu B, et al. Structural properties of hydrolyzed high-amylose rice starch by α-amylase from bacillus licheniformis[J]. J AgricFood Chem, 2011, 59（23）: 12 667-12 673. [27] Wei C, Xu B, Qin F, et al. C-type starch from high-amylose rice resistant starch granules modified by antisense RNA inhibition of starch branching enzyme[J]. J Agric Food Chem, 2010, 58（12）: 7 383-7 388. [28] Sun M M, Lee H J, Abdula S E, et al. Overexpression of starch branching enzyme 1 gene improves eating quality in japonica rice[J]. J Plant Biotechnol, 2013, 40（2）: 88-101. [29] Fujita N, Kubo A, Suh D S, et al. Antisense inhibition of isoamylase alters the structure of amylopectin and the physicochemical properties of starch in rice endosperm[J]. Plant Cell Physiol, 2003, 44（6）: 607-618. [30] Krishnamurthy K, Giroux M J. Expression of wheat puroindoline genes in transgenic rice enhances grain softness[J]. Nature Biotechnol, 2001, 19（2）: 162-166. [31] Chiang C M, Yeh F S, Huang L F, et al. Expression of a bi-functional and thermostable amylopullulanase in transgenic rice seeds leads to autohydrolysis and altered composition of starch[J]. Mol Breed, 2005, 15（2）: 125-143. [32] Su J, Hu C, Yan X, et al. Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice[J]. Nature, 2015, 523（7562）: 602.